20. The inheritance of resistance to gall midge in the rice variety Duokang 1


1) Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640 China
2) Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640 China
3) China National Rice Research Institute, Hangzhou, 310006 China

Most of the gall midge resistant varieties are susceptible to gall midge biotype 4 of Guangdong (Lai et al. 1984). Duokang 1 was derived from Daquiqi of Chinese traditional gall midge resistant variety by hybridization. It can resist to all of four biotypes of Guangdong as Daquiqi. The preliminary study showed that its gall midge resistance is controlled by a single dominant gene and is nonallelic to the Gm1 in W1263 (Pan et al. 1993). This gene is mapped on rice chromosome 4 between RFLP markers RG214 and RG163, and named as Gm6 (t) (Katiyar et al. 1994; Tan et al. 1996). In this report we studied on the genetic relationship of gall midge resistance genes between Duokang1 and some other varieties.

The parental varieties of the crosses and their resistance to biotypes 1 and 4 of Guangdong gall midge are listed in Table 1. Gm2 for resistance to gall midge in Lean 152 was named by Chaudhary et al. (1986), and Gm3 in BG404-1 and gm4 in OB677 by Tomar and Prasad (1992). The F2 and F3 populations of Duokang 1 crossed with Yangshanzhan, Lean 152, BG404-1 and OB677 were used for determining the allelic relationships of resistance gene. The F1 population of Duokang 1/Yangshanzhan//Fengyinzhan was used to determine the linkage relationship of resistance gene. The colonies of gall midge used in test were Guangdong biotypes 1 and 4. Greenhouse screening method was used in the test. Twenty-five days after infesting, the F2 and the three-way cross F1 populations were classified as resistant or susceptible on plant basis, and the F3 lines were classified as homozygous resistant, segregating, and homozygous susceptible on family basis.

Table 2 is the reactions to Guangdong gall midge biotypes 1 of F2 and F3 populations of the crosses. The F2 populations in the crosses of Duokang 1 with Lean 152, BG404-1, and OB677 segregated into a ratio of 15R: 1S. The F3families of these crosses segregated into a ratio of 7R:8Seg:1S, indicating that the dominant resistance gene in Duokang 1 was independent from the genes of Leuangl52, BG404-1 and OB677, and nonallelic to those gall midge resistance genes.

In F2 population of Duokang 1 crossed with Yangshanzhan only one plant was susceptible and one family was segregating in F3 lines (Table 2). Table 3 is the reactions of F1 population of Duokan1/Yangshanzhan//Fengyinzhan to Guangdong gall midge biotypes 1 and 4. All plants of the F1 population were resistant to the biotype 1, and segregated into a ratio of 1R:1S when tested by the biotype 4, indicating the gene of Duokang 1 was non-allelic to, and closely linked with the resistance gene of Yangshanzhan.

Previously, Pan et al. (1992) reported that the gall midge resistance genes of Duokang 1 was nonallelic to the genes of W 1263. Katiyar et al. ( 1994) and Tan et al. ( 1996) named the gene of Duokang 1 for resistance to Guangdong gall midge as Gm6 (t). The resistance of Doukang 1 was different from those resistant varieties in the biotype testing (Table 1). Therefore, we confirmed that Duokang 1 has Gm6.

Mohan et al. (1993) mapped the gene Gm2 of Phalguna on rice chromosome 4, on which Katiyar mapped the Gm6 (t) of Doukang 1. In our study, it was shown that Gm6 of Doukang 1 and Gm2 did not locate on the same chromosome. This contradiction needs to be clarified in future. In this study, it was found that Yangshanzhan, one of Chinese traditional variety, has a dominant resistance gene which is closely linked with Gm6 of Doukang 1.

We thank the supporting for this research from Rice Science Development Fund of China.


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