Strain information
Strain ID TG870
Strain Name Tg(olvas-GFP)
Strain Type Transgenics
Characteristics GFP expression in the germ cells. Transgene: The prmoter region of medaka vasa (olvas) gene and 3'region were inserted into 5'MCS of pEGFP-N1(Clontech) [PDF] and 3'MCS, respectively.
  •    2  Hits.
    • Nishimura T, Herpin A, Kimura T, Hara I, Kawasaki T, Nakamura S, Yamamoto Y, Saito TL, Yoshimura J, Morishita S, Tsukahara T, Kobayashi S, Naruse K, Shigenobu S, Sakai N, Schartl M, Tanaka M. Analysis of a novel gene, Sdgc, reveals sex chromosome-dependent differences of medaka germ cells prior to gonad formation. Development 2014/08/01.141(17).3363-9.

      Ansai S, Ochiai H, Kanie Y, Kamei Y, Gou Y, Kitano T, Yamamoto T, Kinoshita M. Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases. Dev. Growth Differ. 2012/05/31.54(5).546-56.
Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: Tanaka M, Kinoshita M, Kobayashi D, Nagahama Y. Establishment of medaka (Oryzias latipes) transgenic lines with the expression of green fluorescent protein fluorescence exclusively in germ cells: A useful model to monitor germ cells in a live vertebrate. Proc Natl Acad Sci U S A, 98,2544-2549 (2001))
Category Transgenic strains
Organization National Institute for Basic Biology
Live in NIBB
Deposited by National Institute for Basic Biology and Kyoto University
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