|Characteristics||A transgenic medaka expressing a fusion protein between histone H2B and green fluorescence protein (GFP) under the control of a cytomegalovirus (CMV) promoter. Since the nuclei and chromosomes of transgenic medaka cells are labeled with GFP, their morphological changes can be instantly monitored throughout the mitotic cell cycle progression under a fluorescent microscope without any fixation and staining of samples. However, GFP-labeling of nuclei and chromosomes is not successful during early embryonic development until zygotic expression begins and during the meiotic cell cycle progression, because the CMV promoter does not work in these stages. In addition, histone H2B-GFP fusion proteins are expressed in an organ-specific manner; strong and ubiquitous expression occurs in cells comprising the gut and fin, whereas the expression is restricted to certain types of cells in the liver and brain.|
|Special affairs [Address]||
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: Toshiharu Iwai, Shinya Inoue, Tomoya Kotani and Masakane Yamashita (2009) Production of transgenic medaka fish carrying fluorescent nuclei and chromosomes. Zool. Sci., 26: 9-16.)
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||Hokkaido University|
External views of sexually mature male and female CMV-H2B-GFP transgenic medaka, showing the presence of GFP fluorescence in the whole body, with no sex-specific differences. Images taken from the same sample under a bright-field microscope (BF) and a fluorescence microscope (GFP) are shown.