wnt4b-EGFP

Strain information
Strain ID TG1259
Strain Name wnt4b-EGFP
Strain Type Transgenics
Characteristics Wnt4b is a member of wingless-type MMTV integration site family (WNT). A 3.2 kb genomic fragment encompassing the wnt4b promoter region was cloned into the HindIII-SalI sites of the pEGFP-1 vector (CLONTECH).The EGFP expression is observed in the floor plate cells.
Journal
  •    1  Hits.
    • Inohaya K, Takano Y, Kudo A. Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka. Development 2010/06.137(11).1807-13.
Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: Inohaya K, Takano Y, Kudo A. (2010). Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka. Development. 137, 1807-1813.)
Category Transgenic strains
Organization National Institute for Basic Biology
Live in NIBB
Deposited by Tokyo Institute of Technology
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Image

TG1259_transgene-map
A 3.2kb wnt4b promoter was ligated to HindIII and SalI sites of pEGFP1 (Clontech).
Image

TG1259_shh-EGFP_embryo
(A-B) EGFP expresses in the floor plate cells (green). Dorso-lateral view of a day-4 embryo.(A) Bright-field image. (B) Fluorescence image of “A.” (C) Higher magnification of the floor plate cells (green).


/medaka