Strain information
Strain ID TG1230
Strain Name Tg(pnp4a-hs:GFP)
Strain Type Transgenics
Characteristics GFP expression was observed in the eye and abdomen at Juvenal stage. Genotype of the original strain is pnp4a^hs:GFP/gu. Knock-in allele is non-functional and homozygote with transgene shows the less-pigmented iridophores.
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Special affairs [Address] ■ 利用者は、すべての本件刊行物において、次の文献を引用する。
(文献名:WATAKABE, Ikuko, et al. Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering. Zoological Letters (2018) 4:3)
Category Transgenic strains
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by National Institute for Basic Biology
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Knock in at the 5' NTR region of pnp4a gene with Tbait:hs:GFP donor vector.

Transgene map_Tbait-hs-GFP


TG1230_Tg(pnp4a-hs GFP)_embryo
The progenies (4 dpf) of a pnp4a-transgenic founder crossed to an existing gu (MT58) mutant. All GFP-positive F1 fish have less-pigmented eyes and all GFP-negative F1 fish have wild-type pigmentation.