Tg(pKIF5Aa-GFP)

Strain information
Strain ID TG1156
Strain Name Tg(pKIF5Aa-GFP)
Strain Type Transgenics
Characteristics Kinesin family member 5Aa (KIF5Aa) is a gene expressing specifically in neuronal tissues. This strain expresses GFP only in neuronal tissues. Background of the strain was changed from drR to STII. Therefore, the strain is suitable for imaging of neuronal tissues and cells.
Journal
  •    1  Hits.
    • Kawasaki T, Kurauchi K, Higashihata A, Deguchi T, Ishikawa Y, Yamauchi M, Sasanuma M, Hori H, Tsutsumi M, Wakamatsu Y, Yuba S, Kinoshita M. Transgenic medaka fish which mimic the endogenous expression of neuronal kinesin, KIF5A. Brain Res. 2012/10/22.1480.12-21.
Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication:Kawasaki T, Kurauchi K, Higashihata A, Deguchi T, Ishikawa Y, Yamauchi M, Sasanuma M, Hori H, Tsutsumi M, Wakamatsu Y,Yuba S, Kinoshita M., Transgenic medaka fish which mimic the endogenous expression of neuronal kinesin, KIF5A. Brain Res. 2012 Oct 22;1480:12-21.)
Category Transgenic strains
Organization National Institute for Basic Biology
Live in NIBB
Deposited by National Institute of Advanced Industrial Science and Technology (AIST)
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Image

TG1156_Tg(pKIF5Aa-GFP)
GFP was observed clearly in the neuronal tissues later than 5-days after hatch.
Image

TG1156_Tg(pKIF5Aa-GFP)_transgene map
6.6kb of 5'- and 1.9kb of 3'-regulatory region of medaka KIF5A were cloned into 5' MCS and 3' MCS of pEGFP vector (Clontech, Cat:632311), respectively.


/medaka