|Characteristics||The osx:mCherry transgenic embryos express mCherry in pre-mature and mature osteoblasts. (see Renn and Winkler, Dev Dyn 2009). mCherry is expressed in regions of early bone formation and remains highly expressed in mature osteoblasts that mineralize bone matrix.|
|Special affairs [Address]||
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: RENN, Joerg; WINKLER, Christoph. Osterix‐mCherry transgenic medaka for in vivo imaging of bone formation. Developmental Dynamics, 2009, 238.1: 241-248.
TO, Thuy Thanh, et al. Rankl-induced osteoclastogenesis leads to loss of mineralization in a medaka osteoporosis model. Development, 2012, 139.1: 141-150
RENN, Jörg, et al. A col10a1: nlGFP transgenic line displays putative osteoblast precursors at the medaka notochordal sheath prior to mineralization. Developmental biology, 2013, 381.1: 134-143.)
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
|Organization||National Institute for Basic Biology|
|Deposited by||National University of Singapore|
mCherry fluorescence expression in premature and mature osteoblasts in a medaka larvae at 15 dpf. Note strong fluorescence in cleithrum, arches of vertebral bodies and fin rays of caudal fins. For details see Renn and Winkler, Dev Dyn 2009.
TG1134_osx mCherry transgene map
A 4.1-kb fragment (including a putative Runx2 binding site) upstream of the osterix (osx) translational start site from genomic DNA was cloned in front of mCherry into a vector with flanking I-SceI meganuclease restriction sites (for details see Renn and Winkler, Dev Dyn 2009).