|Characteristics||The col10a1:nlGFP transgenic embryos express GFP in osteoblast progenitors and pre-mature osteoblasts (see Renn et al., Dev Biol 2013). At later stages, nlGFP expression is also found in chondrocytes. Note that the nuclear localization signal (nl) is leaky and GFP expression is also observed in cytoplasm.|
|Special affairs [Address]||
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: RENN, Joerg; WINKLER, Christoph. Osterix‐mCherry transgenic medaka for in vivo imaging of bone formation. Developmental Dynamics, 2009, 238.1: 241-248.
TO, Thuy Thanh, et al. Rankl-induced osteoclastogenesis leads to loss of mineralization in a medaka osteoporosis model. Development, 2012, 139.1: 141-150
RENN, Jörg, et al. A col10a1: nlGFP transgenic line displays putative osteoblast precursors at the medaka notochordal sheath prior to mineralization. Developmental biology, 2013, 381.1: 134-143.)
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
|Organization||National Institute for Basic Biology|
|Live in NIBB||○|
|Deposited by||National University of Singapore|
TG1129_col10a1 nlGFP_transgene map
A 5.865kb upstream regulatory region of the medaka col10a1 locus was amplified from genomic medaka DNA using the primers 5′.CCCATGCTTGTTTTCCTGTT-3′ and 5′.TCCAAACAGATCT- GACCTTGC-3′, and cloned in to a meganuclease vector with GFP followed by a nuclear localization signal (for details see Renn et al., Dev Biol 2013).
TG1129 col10_nlGFP 15dpf
nlGFP expression in a Medaka embryo at 15 dpf. nlGFP expression can be found in cranial skeletal elements, around the centra of the vertebral bodies, in the neural and hemal arches, and in the fin rays of the caudal fin. For more details, please see Renn et al., Dev Biol 2013.