Containing single copy attP motif for phiC31-mediated transgenesis.
The transgene is inserted at Chr8: 25,436,300-25,436,307, intron of abat gene (ENSORLG00000019754).
The zhsp70-mCherry fragment is also integrated as a TG marker, which causes basal RFP expression in lens and inducible RFP expression in the whole body with a heat shock treatment. This marker can be removed by FLP mRNA injection after phiC31 mediated recombination.
|Special affairs [Address]||
■ The RECIPIENT shall include the name(s) of following researcher(s) as co-authors in the first PUBLICATION. (Name(s) of researcher(s): Tokiro Ishikawa and Satoshi Ansai)
■ The RECIPIENT shall include the names of following researchers as co-authors in the first PUBLICATION.
(Names of researchers: Tokiro Ishikawa and Satoshi Ansai).
The RECIPIENT should make a prior contact with Tokiro Ishikawa (email@example.com) and Satoshi Ansai (firstname.lastname@example.org) before submitting the first PUBLICATION and receive approval for the submission.
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||Kyoto University|
attP, Pzhsp70, mCherry, SV40 polyA or FRT is placed in this order.