|Characteristics||Double transgenic line in which medaka myeloid cells express simultaneously Ruby-tagged Lifeact (RFP.Lifeact), which detects polymerized actin, and the end-binding protein 3 (EB3) fused to EGFP, which binds to microtubule plus-end tips and also allows visualization of the microtubule organizing center (MTOC). The heart-specific cMLC2::ECFP expression cassette allows a preliminary screening of transgenic animals. Transgene: TG(cmlc2::ECFP FmpoP::EB3-EGFP/cmlc2::ECFP FmpoP::RFP-Lifeact)|
|Special affairs [Address]||
■The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication:The PAR complex controls the spatiotemporal dynamics of F-actin and the MTOC in directionally migrating leukocytes. Crespo CL, Vernieri C, Keller PJ, Garrè M, Bender JR, Wittbrodt J, Pardi R. J Cell Sci. 2014 Oct 15;127(Pt 20):4381-95.)
■The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||San Raffaele Scientific Institute|
EB3/Lifeact expression in the tail fin of TG(FmpoP::EB3-EGFP/FmpoP::RFP-Lifeact).
YFP expression in TG(FmpoP_mYFP)
Localization of membrane-tethered YFP (memYFP)-positive cells in TG(FmpoP::memYFP) (white arrows) (Grabher et al., 2007). EB3/Lifeact double positive cells in TG(FmpoP::EB3-EGFP/FmpoP::RFP-Lifeact) show exactly the same distribution pattern as memYFP-positive cells in TG(FmpoP::memYFP). Adapted from Grabher et al., 2007.