Loosli F, Del Bene F, Quiring R, Rembold M, Martinez-Morales JR, Carl M, Grabher C, Iquel C, Krone A, Wittbrodt B, Winkler S, Sasado T, Morinaga C, Suwa H, Niwa K, Henrich T, Deguchi T, Hirose Y, Iwanami N, Kunimatsu S, Osakada M, Watanabe T, Yasuoka A, Yoda H, Winkler C, Elmasri H, Kondoh H, Furutani-Seiki M, Wittbrodt J. Mutations affecting retina development in Medaka. Mech Dev 2004/07/01.121(7-8).703-14.
■The RECIPIENT shall include the name(s) of following researcher(s) as co-authors in the first PUBLICATION. (Name(s) of researcher(s): Makoto Furutani-Seiki and Hisato Kondoh)
Mutant of embryogeneses
National Institute for Basic Biology
Frozen sperm in NIBB
ERATO Kondoh Differentiation Signaling Project, Japan Science and Technology Agency
ERATO Kondoh Project
PGCs formed tight bilateral clumps in wild-type embryos at st. 27. In the akatsuki (akuj22-15A) mutant embryos, PGCs were variably scattered in the trunk with a fraction distributed along the midline. It was noted that these mutant embryos also showed various degrees of defects in the structures along the midline, such as cyclopic eyes, small tecta and hindbrain, a thin notochord and a narrow somitic mesoderm at st. 27. The extent of PGC scattering generally correlated with the severity of midline defects. aku mutation was a recessive and homozygosity caused embryonic lethality.
○side view (lateral)
○anterior to left
●anterior to top
○anterior to right
○dorsal to top
○dorsal to right
Caption / Comment
Whole-mount in situ hybridization using the olvas probe at st. 27. In the wild-type embryo (A; top) PGCs are clustered in the ventrolateral regions of the trunk. The aku mutant embryos exhibit various degrees of abnormal distribution of PGCs (B, C). PGCs are severely (C) and more moderately (B) scattered in the medial part of the trunk including the midline region of the embryos. Some PGCs tend to form clumps (indicated by arrowheads in B and C). Two clumps of PGCs in B (indicated by arrowheads) are formed around the regions where PGCs normally cluster. Broken lines in A indicate the lateral edges of the body.