sp7^TAL1

Strain information
Strain ID MT1240
Strain Name sp7^TAL1
Strain Type Mutants
Characteristics A 7 bp was deleted in exon 2 of sp7 (specificity protein 7) gene, which is also known as osterix (osx), by the TALEN genome editing system. This mutation is expected to introduce frame-shift mutation, resulting in truncation of the C-terminus of the sp7 protein, including the zinc-finger domain. The mutation position of sp7^TAL1 (MT1240) is different from that of sp7^TAL2 (MT1241). This mutant showed severe defects in the formation of skeletal structures. In particular, the neural and the hemal arches were not formed, although the chordal centra were formed. There were no differences in the skeletal phenotypes between the sp7^TAL1 (MT1240) and sp7^TAL2 (MT1241) mutants. The homozygous mutant is non-viable and died around 14 days after hatching.
Journal
  •    1  Hits.
    • Azetsu Y, Inohaya K, Takano Y, Kinoshita M, Tasaki M, Kudo A. The sp7 gene is required for maturation of osteoblast-lineage cells in medaka (Oryzias latipes) vertebral column development. Dev. Biol. 2017/11/15.431(2).252-262.
Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: Azetsu, Y., Inohaya, K., Takano, Y., Kinoshita, M., Tasaki, M., & Kudo, A. (2017). The sp7 gene is required for maturation of osteoblast-lineage cells in medaka (Oryzias latipes) vertebral column development. Developmental biology, 431(2), 252-262.)
Category Mutants created by genome editing
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by Tokyo Institute of Technology
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Document (PDF) Genotyping protocol_MT1240_sp7^TAL1


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