A 16 bp deletion was generated by TALEN method in exon 2 of sox10a gene on LG1. The target spacer sequence of TALEN was GAGGCCATTCATCGAG. The sox10a gene encodes a HMG type transcription factor SoxE family, and is expressed in the neural crest cells.
Sox10a plays an important role for pigment cell develpment. The homozygous larvae show reduction in melanophore, iridophore and xanthophore numbers.
The original background is Nagoya, with wild-type body color. Homozygote sox10a mutant cannot survive beyond hatching.
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■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
|Category||Mutants created by genome editing|
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||Nagoya University|
sox10 mutants_9 dpf
Medaka wild-type (WS3 Nagoya) 9 dpf larvae show a pigment pattern as having horizontal melanophore stripes and yellowish in-betweens (xanthophores) with leucophores and iridophores (not apparent in the photo). The sox10b-/- (MT1192 sox10b^N108S) larvae show a normal pigment pattern. The sox10a-/- (MT1196 sox10a^E2del16) larvae show severe reduction in melanophore, iridophore and xanthophore numbers. The sox10a-/-sox10-/- double mutants (MT1189 sox10a^E2del16;sox10b^N108S) lack all chromatophores, except that leucophores on the head are retained.
Lateral views. Anterior to the left.
|Document (PDF)||Genotyping protocol_sox10a^E2del16|