This mutant, isolated by TILLING screening, has a single nucleotide substitution in exon 1 of sox10b gene. The mutation causes an amino acid substitution (N to S) in the conserved sequence of HMG box.
The homozygotes look normal and sometimes survive to adulthood.
When crossed to sox10a mutant, sox10b mutant enhances the reduction of pigment cells in sox10a mutant.
|Special affairs [Address]||
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: Taniguchi Y, Takeda S, Furutani-Seiki M, Kamei Y, Todo T, Sasado T, Deguchi T, Kondoh H, Mudde J, Yamazoe M, Hidaka M, Mitani H, Toyoda A, Sakaki Y, Plasterk RH, Cuppen E. (2006) Generation of medaka gene knockout models by target-selected mutagenesis. Genome Biol. 7(12):R116)
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
|Category||Mutants created by TILLING|
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||Nagoya university|
sox10 mutants_9 dpf
Medaka wild-type (WS3 Nagoya) 9 dpf larvae show a pigment pattern as having horizontal melanophore stripes and yellowish in-betweens (xanthophores) with leucophores and iridophores (not apparent in the photo). The sox10b-/- (MT1192 sox10b^N108S) larvae show a normal pigment pattern. The sox10a-/- (MT1196 sox10a^E2del16) larvae show severe reduction in melanophore, iridophore and xanthophore numbers. The sox10a-/-sox10-/- double mutants (MT1189 sox10a^E2del16;sox10b^N108S) lack all chromatophores, except that leucophores on the head are retained.
Lateral views. Anterior to the left.
|Document (PDF)||Genotyping protocol_MT1192_sox10b^N108S|