A 10 bp insertion was generated by TALEN method in exon 1 of sox10a gene on LG1. The target sequences of TALEN were specific target: 5'-TTCTGAGTCGGAGCTGAGC-3' (left, sense) and 5'-TGAGAGTGAGTGACCGTCC-3' (right, antisense). The sox10a gene encodes a HMG type transcription factor SoxE family, and is expressed in the neural crest cells.
Sox10a plays an important role for pigment cell develpment. The homozygous larvae show reduction in melanophore, iridophore and xanthophore numbers, and do not survive beyond hatching.
The original background is Nagoya, with wild-type body color.
|Notes||Please note that we will use OK-Cab (MT830) for artificially insemination with frozen sperm of this strain.|
|Special affairs [Address]||
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: Nagao Y, Takada H, Miyadai M, Adachi T, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H. (2018) Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish. PLoS Genet, 14: e1007260.)
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
|Category||Mutants created by genome editing|
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||Nagoya University|
|Document (PDF)||Genotyping protocol_MT1187_sox10a^E1ins10|