|Characteristics||As a result of TILLING screening, TILLING_MUTANT(ID:1325) (TA1325) was identified as a mutant with a single nucleotide substitution in sox5 exon13. Homozygous mutant embryos and larvae have excess leucophores and lack xanthophores. Similarly, homozygous adult fish have excess leucophores, but unlike at earlier stages, they have xanthophores significantly reduced in number. Formation of melanophores and iridophores is not affected in this mutant. This strain is OK-Cab background.|
|Special affairs [Address]||
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library. Ishikawa T, Kamei Y, Otozai S, Kim J, Sato A, Kuwahara Y, Tanaka M, Deguchi T, Inohara H, Tsujimura T, Todo T. BMC Mol Biol. 2010 Sep 15;11:70.
TANIGUCHI, Yoshihito, et al. Generation of medaka gene knockout models by target-selected mutagenesis. Genome biology, 2006, 7.12: R116.
NAGAO, Yusuke, et al. Sox5 functions as a fate switch in medaka pigment cell development. PLoS Genet, 2014, 10.4: e1004246.)
|Category||Mutants created by TILLING|
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||Nagoya University|
MT1048-1049 sox5 mutant
The mutant hatchlings exhibit excess and ectopic leucophores and no xanthophore. Leucophores are shown in the darkfield image (left panel). Xanthophores are not detectable under UV light (right panel).
|Document (PDF)||MT1048_Genotyping protocol|
|Document (PDF)||sox5 cDNA seq|