|Characteristics||The medaka mutant of cyp19a1 (ovarian aromatase) screened by ENU-based gene-driven mutagenesis library of medaka. In this mutant, one amino acid in the cyp19a1 open reading frame altered the stop codon. In the K164X strain, lysine in exon 3 (AAA) was mutated to the stop codon TAA. In the Q183X strain, glutamine in exon 4 (CAG) was altered to the stop codon TAG. The K164X and Q183X strains displayed the same phenotype. In these mutant, the activity of cyp19a1 was lost and thus estrogens in the gonad were extremely reduced. In XX mutants, gonads first differentiated into normal ovaries containing many ovarian follicles that failed to accumulate yolk. Abnormal proliferation of the granulosa cell layers of the ovarian follicles was observed in ovaries. Subsequently, ovarian tissues underwent extensive degeneration, followed by the appearance of testicular tissues on the dorsal side of ovaries.|
|Category||Mutants created by TILLING|
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||National Institute for Basic Biology, Ehime University|
Phenotype of the medaka mutant of cyp19a1 (ovarian aromatase). Genetic female (XX) cyp19a1 mutant medaka developed into females displaying ovaries that failed to accumulate yolk (A and C). (B) Thereafter, the morphology of anal fins gradually changed into the male type. (D) Ovarian follicles degenerated. (D) High magnification image of the area marked with a rectangle in C. In parts of degenerating gonads, spermatogenic-like tissue was observed (arrowheads). Scale bars: 1mm in A and B, 100 µm in C and D, 10 µm in E.
|Document (PDF)||Genotyping protocol_MT1032_cyp19a1(K164X)|