|Characteristics||Tryptophan hydroxylase 2 (tph2) gene was disrupted in a d-rR strain using TALENs. This mutant carries a 13-bp deletion in the 6th exon of the tph2 gene. Homozygous mutants lost a neurotransmitter serotonin (5-HT) in tph2-expressing neurons whose axons are projected from raphe nuclei in the brain stem to the whole brain. Homozygous mutants showed behavioral alterations, such as an increased freezing response when the fish were a novel tank, and curved vertebral columns.|
Please note that we will use OK-Cab (MT830) for artificially insemination with frozen sperm of this strain.
Homozygote is normally fertile but starts to show curved vertebral columns around their sexual maturation (approximately 2 months post-hatching).
|Special affairs [Address]||
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: Ansai, S., Hosokawa, H., Maegawa, S., Naruse, K., Washio, Y., Sato, K., & Kinoshita, M. (2017). Deficiency of Serotonin in Raphe Neurons and Altered Behavioral Responses in Tryptophan Hydroxylase 2-Knockout Medaka (Oryzias latipes). Zebrafish.)
|Category||Mutants created by genome editing|
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||Kyoto University|
|Document (PDF)||Genotyping protocol_MT1074.pdf|