ctsk:nlGFP

Strain information
Strain ID TG1132
Strain Name ctsk:nlGFP
Strain Type Transgenics
Characteristics The ctsk:nlGFP transgenic embryos express GFP in bone resorbing osteoclasts (see To et al., Comp Biochem Physiol C 2015 ). In early embryos, it is also expressed in ctsk positive cells in the head and in the tail fin, which are mesenchymal cells but not osteoclasts. First osteoclasts in the vertebral column appear at 21 days post fertilization. Note that the nuclear localization signal (nl) is leaky and GFP expression is also observed in cytoplasm.
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Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: TO, Thuy Thanh, et al. Rankl-induced osteoclastogenesis leads to loss of mineralization in a medaka osteoporosis model. Development, 2012, 139.1: 141-150
TO, Thuy Thanh, et al. An adult osteopetrosis model in medaka reveals the importance of osteoclast function for bone remodeling in teleost fish. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2015, 178: 68-75.)
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
Category Transgenic strains
Organization National Institute for Basic Biology
Live
Deposited by National University of Singapore
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Image

TG1132-ctsk-nlGFP 3 wpf
Lateral view of ctsk:nlGFP medaka at 3 weeks post fertilization. GFP expression is seen in osteoclasts of hemal and neural arches in the vertebral bodies, and in fin rays. Expression at base of fins and in skull might be in cathepsinK-positive cells that are likely not osteoclasts.
NOTE that GFP expression is not restricted to nuclei but also found in cytoplasm.
Document (PDF) TG1132 ctsk-nlGFP plasmid map


/medaka