Strain information
Strain ID TG958
Strain Name ci-Tg(Actb-GH:GFP)
Strain Type Transgenics
Characteristics Fish of this strain grow fast and big, because of ectopic overexpression of the growth hormone. The transgene also expresses GFP as a reporter. Transgene: Medaka beta-actin promoter was ligated to Medaka growth hormone-IRES-GFP fusion gene in pGEM-T easy vector (Promega). An internal ribosome entry site (IRES)” from the pIRES-hrGFP-1a vector (Stratagene), “a GFP open-reading frame” from the phrGFP II-1 vector (Stratagene), and “SV40 polyA signals” from the pDsRed2-1 vector (Clontech).
Notes Females are severely sterile. Males are incompletely sterile. Therefore, this line should be maintained by corssing heterozygous males with ci females. We use OK-Cab (MT830) embryos in artificially- insemination. Please note that we will use OK-Cab (MT830) for artificially insemination with frozen sperm of this strain.
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Special affairs [Address] ■The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
Category Transgenic strains
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by Japan Women's University
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Adult fish of the transgenic ci-TG(Actb-GH:GFP) (top) and its host strain ci (bottom). The transgenic fish is twice as heavier as the host fish because of the overexpressed GH. Scale bars; 0.5 mm.