Vacuolar-type H(+) ATPases (V-ATPases) are multimeric protein complexes that play a universal role in the acidification of intracellular compartments in eukaryotic cells. In a ENU screen, we have isolated the recessive medaka mutation tintachina (tch), which carries an inactivating modification of the conserved glycine residue (G75R) of the proton pump subunit atp6v1Ba/vatB1. Mutant embryos show normal morphology but penetrant pigmentation defects from stage 27 on. As development proceeds, embryos display progressive brain apoptosis and finally die before hatching.
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: The medaka mutation tintachina sheds light on the evolution of V-ATPase B subunits in vertebrates. Müller C, Maeso I, Wittbrodt J, Martínez-Morales JR. Sci Rep. (2013) 3:3217. )
Mutants created by TILLING
National Institute for Basic Biology
Frozen sperm in NIBB
Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de Olavide