|Characteristics||This strain expresses EGFP and dominant negative type G-protein alpha subunit (Gi2S47C) in a subset of taste bud cells under the control of medaka phospholipase C beta 2 (mfplcb2) transcriptional regulatory region.|
|Special affairs [Address]||
■ The RECIPIENT shall include the name(s) of following researcher(s) as co-authors in the first PUBLICATION.
(Name(s) of researcher(s): Aihara Y, Yasuoka A, Iwamoto S, Yoshida Y, Misaka T, Abe K. Construction of a taste-blind medaka fish and quantitative assay of its preference-aversion behavior. 2008 Genes Brain Behav. 7(8):924-32)
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||The University of Tokyo|
Generation of transgenic medaka fish expressing a dominant-negative form of the Galphai2 subunit in taste receptor cells. (a) The construct contains two medaka plc-beta2 promoters that regulate rat galphai2S47C mutant and gfp genes. The two genes are in an inverted configuration with an I-Sce I meganuclease site at each 3′ end. Scale bar: 1 kbp. (b) The transgenic fish express green fluorescent protein (GFP) in the regions where taste buds are distributed. GFP was detected in the lip (left panel) and pharyngeal regions (right panel) of the transgenic fish (10 dpf). Images were obtained by overlaying fluorescence images on bright-field images. Scale bar: 200 micro-m. (c) Coexpression of the transgenes and endogenous plc-beta2 gene in the taste bud cells. In each experiment, fluorescence microscopy (panels in the left column) and in situ hybridization analyses (panels in the center column) were performed using the same section (upper or lower panels). The GFP signals were detected only in the cells expressing the endogenous plc-beta2 gene (upper panels). The cells expressing GFP also expressed rat Galphai2 mRNA (lower panels). Scale bar: 10 micro-m.