|Characteristics||This strain expresses EGFP and recombinant wheat germ agglutinin (WGA) in a subset of taste bud cells under the control of medaka phospholipase C beta 2 (mfplcb2) transcriptional regulatory region. WGA was transported trans-synaptically resulting in the labeling of brain regions related to gustatory transduction.|
|Special affairs [Address]||
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication:Ieki T, Okada S, Aihara Y, Ohmoto M, Abe K, Yasuoka A, Misaka T. Transgenic labeling of higher-order neuronal circuits linked to phospholipase C-β2-expressing taste bud cells in medaka fish. 2012 J Comp Neurol. 521(8):1781-802)
|Organization||National Institute for Basic Biology|
|Frozen sperm in NIBB||○|
|Deposited by||The University of Tokyo|
Generation of transgenic fish expressing the WGA gene in a subpopulation of taste bud cells. A: Design of the transgene. Abbreviations: 50-mfplcb2-1.6k, 50-transcriptional regulatory region of the mfplc-b2 gene (Aihara et al., 2007); bgl, rabbit b-globin gene intron with splicing sites; WGA, coding sequence for modified wheat germ agglutinin (Yoshihara et al., 1999); open triangles, I-Sce I recognition site for the facilitation of transgenesis (Thermes et al., 2002); Kanr, kanamycin resistance gene; pA, SV40 polyadenylation signal; EGFP, enhanced green fluorescent protein gene. B: In situ hybridization of gustatory tissue sections (lip, gill raker, and pharynx) from 3-month-old transgenic (Tg) or wild-type (WT) fish using an antisense riboprobe for WGA mRNA. Signals were detected in the taste bud cells of Tg fish (upper panels) but not in WT fish (lower panels). C: Results of double-label fluorescent in situ hybridization of taste bud cell sections from 3-month-old Tg fish. WGA mRNA colocalized with endogenous mfplc-b2 mRNA (upper panels) but not with the medaka ortholog of the acid-sensing cation channel, mfpkd2l1 mRNA (lower panels). D: Distribution of WGA protein in the taste buds. Sections of 3-month-old Tg medaka were subjected to in situ hybridization using a WGA antisense riboprobe (upper panels) or an mfpkd2l1 antisense riboprobe (lower panels) followed by anti-WGA immunostaining. The WGA protein (purple signal) was not detected in mfpkd2l1-positive cells (green signal). Panels are rep- resentatives of experiments performed using three Tg fish from each Tg line.