Hd-rRII1-Chromosome10Kaga

Strain information
Strain ID IB960
Strain Name Hd-rRII1-Chromosome10Kaga
Strain Type Inbred strains
Characteristics Kaga chromosome 10 transferred onto a Hd-rRII1 background by speed congenic method. The Kaga chromosome 10 was confirmed by genotyping using four polymorphic markers, MID1011, MID1024, MID1014, and MID1015. PCRs were performed on a T1 thermocycler (Biometra, Göttingen, Germany) by denaturation at 95°C for 2 min, followed by 40 cycles of amplification at 95°C for 30 sec, 55°C for 30 sec, and 72°C for 30 sec, followed by an additional elongation at 72°C for 1 min after the last cycle. Refer to Hd-rRII1-Chromosome10Kaga Genotyping protocol for marker sequence and band size.
Journal
Category Consomic strains
Organization National Institute for Basic Biology
Live in NIBB
Frozen sperm in NIBB
Deposited by National Institute for Basic Biology
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Image


IB960_Genotyping Protocol


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