T5-Tg(olphsp70.1-hRluc-Venus)

Strain information
Strain ID TG908
Strain Name T5-Tg(olphsp70.1-hRluc-Venus)
Strain Type Transgenics
Symbol unkown
Characteristics Inducible expresssion of hRluca-Venus was observed in embryos under the fluorescence microscope. After heat shock (42C, 1min), adult fishes show Venus fluorescence in most tissues and organs, except for germ cells. A transgenic embryos and adult fishes expressed Venus in the lens without heat shock. Transgene: 3.4kb medaka heat shock promoter was ligated to codon-humanized Renilla reniformis luciferase (Promega) - Venus (Nagai et al., 2002) fusion gene in pGL4.70 [hRluc] (Promega).
Journal
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Category Transgenic strains
Organization National Institute for Basic Biology
Live
Deposited by The University of Tokyo
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Image

TG908_T5-Tg(olphsp70.1-hRluc-Venus)_embryo
Heat shock (42C for 10 min) induced Venus expression in embryos. Images taken from the same sample under a fluorescence microscope (Left panel) and a bright-field microscope (Right panel) are shown.
Image

TG908_T5-Tg(olphsp70.1-hRluc-Venus)_adult
Heat shock (42C for 10 min) induced Venus expression in adult fish throughout the entire body. Images taken from the same sample under a bright-field microscope (Left panel) and a fluorescence microscope (Right panel) are shown. The upper fish is transgenic and the lower fish is wild type.
Image

TG908_T5-Tg(olphsp70.1-hRluc-Venus) transgene map



/medaka