This strain was generated by crossing Tg(TRE2-Pkd2deltaC-EGFP and Tg(EF-1alpha-A-rtTA.). Doxycycline (Dox) induce the expression of Medaka polycystic kidney disease 2 (Pkd2) delta C-EGFP. Transgene: Medaka EF-1α-A promoter, rtTA, Medaka polycystic kidney disease 2 (Pkd2) gene, EGFP, pTRE2hyg.
National Institute for Basic Biology
Frozen sperm in NIBB
Hokkaido University and Nagoya University
TG839_Tg(Tet On Pkd2deltaC-EGFP) Histological analysis (HE staining) of adult medaka at aged four months in (rtTA/-,Pkd2ΔC/-) Tet-On Pkd2ΔC-EGFP.
TG839_Tg(Tet On Pkd2deltaC-EGFP)2
The homozygous transgenic (Tet On Pkd2deltaC-EGFP) medaka at 5 months after hatching has severe kidney cysts in hemotoxyline-eosin-stained section (original magnification x40). Arrows indicate cysts. * indicate uretea.