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myf5-EGFP
| Strain information | |
|---|---|
| Strain ID | TG1255 |
| Strain Name | myf5-EGFP |
| Strain Type | Transgenics |
| Characteristics | myf5 (myogenic factor 5) is a basic helix loop helix transcription factor and acts as a myogenic regulatory factor. A fosmid clone (GOLWFno481_a14) including the myf5 gene, which was identified from the Medaka ensemble database, was used for homologous recombination. The EGFP-kanamycin cassette was recombined into exon 1 of the medaka myf5 gene. The medaka Cab line was used for establishment of the myf5-EGFP transgenic medaka. The EGFP expression is observed in muscle cells. |
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| Special affairs [Address] | ■ The RECIPIENT should make a prior contact with Keiji Inohaya (kinohaya@bio.titech.ac.jp), and arrange for collaborative research or offer conditions with a written form of consent. Then, the RECIPIENT shall make a request for this strain to NBRP Medaka at NIBB. |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Tokyo Institute of Technology |
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| Image |
TG1255_transgene-map |
| Image |
TG1255_myf5-EGFP_larva (A) Lateral view of a just hatched larva. EGFP-expression is observed in the muscle cells. (B) Higher magnification of the trunk region of A. |
