shh-EGFP

Strain information
Strain ID TG1257
Strain Name shh-EGFP
Strain Type Transgenics
Characteristics shha (sonic hedgehog a) is a member of the hedgehog gene family. A fosmid clone (GOLWFno139_g12) including the shha gene, which was identified from the Medaka ensemble database, was used for homologous recombination. The EGFP-kanamycin cassette was recombined into exon 1 of the medaka shha gene. The medaka Cab line was used for establishment of the shha-EGFP transgenic medaka. The EGFP expression is mainly observed in notochord.
Journal
Special affairs [Address] ■ The RECIPIENT should make a prior contact with Keiji Inohaya (kinohaya@bio.titech.ac.jp), and arrange for collaborative research or offer conditions with a written form of consent. Then, the RECIPIENT shall make a request for this strain to NBRP Medaka at NIBB.
Category Transgenic strains
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by Tokyo Institute of Technology
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Image

TG1257_transgene-map

Image

TG1257_shh-EGFP_embryo
(A) Dorsal view of a day-2 embryo. EGFP-expression is observed in the notochord. (B) Lateral view of a day-4 embryo.
Image

TG1257_shh-EGFP_larva
Lateral view of a just hatched larva. (B) Higher magnification of the notochord of A. (C) Higher magnification of the tail region of A. EGFP is weekly expressed in the caudal fin (arrowheads).


/medaka