Tg[nr5a1-hs:lRl-GFP]

Strain information
Strain ID TG1183
Strain Name Tg[nr5a1-hs:lRl-GFP]
Strain Type Transgenics
Characteristics LoxP-DsRed-loxP-GFP transgenic medaka for nr5a1 (nuclear receptor subfamily 5, group A, member 1) loci.
Journal
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Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: WATAKABE, Ikuko, et al. Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering. Zoological Letters (2018) 4:3)
Category Transgenic strains
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by National Institute for Basic Biology
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Image

TG1183_fig-(DsRed-expression)
DsRed expression in hypothalamus, interrenal glands and gonad
Image

Transgene map_[olhs-loxP-DsRed-loxP-GFP]
Donor plasmid contains Tbait (a bait sequence derived from the mouse Tet1 gene), medaka hsp70.1 promoter (ca. 0.8kb), loxP, DsRed, polyA, loxP, EGFP, polyA.


/medaka