col10a1:nlGFP/rankl:HSE:CFP

Strain information
Strain ID TG1136
Strain Name col10a1:nlGFP/rankl:HSE:CFP
Strain Type Transgenics
Characteristics This line is double transgenic which derived from col10a1:nlGFP(TG1129)and rankl:HSE:CFP(TG1135). Double transgenic expressing the osteoclast-inducing factor Receptor Activator of Nuclear Factor kB ligand (RANKL) and CFP under bidirectional control of a heat shock-inducible promoter and labelling collegen10 (col10α1) expressing osteoblast precursors with nlGFP.
Upon Rankl induction at 9dpf (incubating embryos at 39°C for 2 hours), in rankl:HSE:CFP larvae, a severe loss in mineralized matrix of most embryos overexpressing Rankl resulting in an osteoporotic phenotype can be observed; with losses varying in extent (Fig. 7A-D. To, TT. et al.,2012).
Journal
Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: RENN, Joerg; WINKLER, Christoph. Osterix‐mCherry transgenic medaka for in vivo imaging of bone formation. Developmental Dynamics, 2009, 238.1: 241-248.
TO, Thuy Thanh, et al. Rankl-induced osteoclastogenesis leads to loss of mineralization in a medaka osteoporosis model. Development, 2012, 139.1: 141-150
RENN, Jörg, et al. A col10a1: nlGFP transgenic line displays putative osteoblast precursors at the medaka notochordal sheath prior to mineralization. Developmental biology, 2013, 381.1: 134-143.)
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
Category Transgenic strains
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by National University of Singapore
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