T5-Tg(avt-egfp)
Strain information | |
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Strain ID | TG976 |
Strain Name | T5-Tg(avt-egfp) |
Strain Type | Transgenics |
Characteristics | This transgenic line derived from T5 strain expresses EGFP under the control of the translation of the arginine vasotocin(avt) gene. EGFP signals are detected in brain of 2-dpf embryo, and continuously shown in adult brain. Until early stage of hatched juveniles, EGFP signals can be detected under a fluorescent microscope without fixation or dissection. In adult brain, EGFP signals are able to be detected in preoptic area and pituitary in live brain slices and/or frozen sections. The cells with EGFP signals are confirmed to be AVT neurons by double-immunohistochemistry using anti-AVT and anti-EGFP antibodies. |
Notes | Please note that we will use OK-Cab (MT830) for artificially insemination with frozen sperm of this strain. |
Journal
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Category | Transgenic strains |
Organization | National Institute for Basic Biology |
Frozen sperm in NIBB | ○ |
Deposited by | Kindai University |
Order | |
Image |
TG976_T5-Tg(avt-egfp)_map The avt-GFP construct is the modified fosmid clone. The EGFP-poly(A)-Km fragment (Kimura et al., 2006) was inserted at the translation initiation site of avt gene in a fosmid clone (GOLWFno393_p20). |
Image |
TG976_T5-Tg(avt-egfp)_6 dpf At 6 dpf, several pairs of EGFP signals are shown between telencephalon and optic tectum, and at the central part of optic tectum. |
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TG976_T5-Tg(avt-egfp)_adult brain In the oblique sections of adult brain, somata and fibers with EGFP signals are shown in preoptic area, anterior and posterior hypothalamus. |
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TG976_T5-Tg(avt-egfp)_adult pituitary EGFP signals are also shown in adult pituitary (sagittal section). |