|
acp5-EGFP
| Strain information | |
|---|---|
| Strain ID | TG1248 |
| Strain Name | acp5-EGFP |
| Strain Type | Transgenics |
| Characteristics | acp5 (acid phosphatase 5, tartrate resistant), also known as TRAP, is a glycosylated monomeric metalloprotein enzyme. acp5/TRAP is used as a differentiation marker of osteoclasts. A fosmid clone (GOLWFno144_g15) including the acp5 gene, which was identified from the Medaka ensemble database, was used for homologous recombination. The EGFP-kanamycin cassette was recombined into exon 1 of the medaka acp5 gene. The medaka Cab line was used for establishment of the acp5-EGFP transgenic medaka. The EGFP expression is observed in putative osteoblasts. |
Journal
|
|
| Special affairs [Address] | ■ The RECIPIENT should make a prior contact with Keiji Inohaya (kinohaya@bio.titech.ac.jp), and arrange for collaborative research or offer conditions with a written form of consent. Then, the RECIPIENT shall make a request for this strain to NBRP Medaka at NIBB. |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Tokyo Institute of Technology |
| Order |
|
| Image |
TG1248_transgene-map |
| Image |
TG1248_acp5-EGFP_Larva Lateral view of tail region of ca. 8.5 mm larva. EGFP-expression is observed in putative osteoclast cells. The putative osteoclast cells are located on vertebral arches (arrowheads) and anal fin (arrow). |
