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MMP9-DsRed
| Strain information | |
|---|---|
| Strain ID | TG1357 |
| Strain Name | MMP9-DsRed |
| Strain Type | Transgenics |
| Characteristics | MMP9-DsRed transgenic medaka line marks MMP9 positive macrophage and osteoclast. |
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| Special affairs [Address] |
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same. |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Showa University |
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| Image |
TG1357_MMP9-DsRed_transgene map.png A 3.2-kb upstream regulatory region of the medaka mmp9 gene was amplified by using the following primers: 5’ -GCGAAGCTTCATGTTGCTAAGTCTCAGAGTC-3’ (forward) and 5’ -GCGAATTCTTTGAGATCTAATGTGGACTAGTG-3’ (reverse). The amplified fragments were cloned into the TA cloning vector. We then digested the 5′ -half fragment with HindIII and EcoRI, and subcloned it into the HindIII/EcoRI site of the I-Sce1 backbone vector, which was subsequently inserted into DsRed-Express/SV40polyA (Clontech) at the HindIII/AflII sites in the pBSKI2 vector (AMAGEN). |
| Image |
TG1357 MMP9-DsRed DsRed is expressed in osteoclast-like cells around the bone. |
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