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Nagoya;TgBAC(pax7a:loxp-dsred-loxp-sox5^WT-2A-venus)
| Strain information | |
|---|---|
| Strain ID | TG1211 |
| Strain Name | Nagoya;TgBAC(pax7a:loxp-dsred-loxp-sox5^WT-2A-venus) |
| Strain Type | Transgenics |
| Characteristics |
DsRed fluorescence is driven by pax7a promoter. Once recombination is induced by cre at the loxp sites, sox5 (WT) becomes expressed there. ml-3;TgBAC(pax7a:loxp-dsred-loxp-sox5^WT-2A-venus) (TG1210) was crossed with Nagoya so that ml-3 mutant allele at the sox5 locus became replaced by wild-type allele. |
| Notes | Please note that we will use OK-Cab (MT830) for artificially insemination with frozen sperm of this strain. |
Journal
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| Special affairs [Address] |
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: Nagao Y, Takada H, Miyadai M, Adachi T, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H. (2018) Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish. PLoS Genet, 14: e1007260.) ■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same. |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Nagoya University |
| Order |
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| Image |
Transgene map_TgBAC(pax7aloxp-dsred-loxp-sox5-2A-venus) |
| Image |
TG1211 Nagoya TgBAC(pax7aloxp-dsred-loxp-sox5^WT-2A-venus) Tg(hsp70-cre) When sox5+/+ WT embryos obtained from crossing the transgenic fish Nagoya (sox5+/+);TgBAC(pax7a:loxp-dsred-loxp-sox5^WT) with Tg(hsp70:cre) were heat shocked, Dsred became barely detectable. Leucophores (Leu) were significantly reduced in the hatchlings treated with heat shock (40℃, 40min) compared with those without heat shock (control) whereas melanocyte formation was not altered by heat shock. |
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