sakura^jR10-4A

Strain information
Strain ID MT417
Strain Name sakura^jR10-4A
Strain Type Mutants
Symbol sak^jR10-4A
Characteristics Abnormal liver development; abnormal PGC distribution
Journal
Special affairs [Address] ■The RECIPIENT shall include the name(s) of following researcher(s) as co-authors in the first PUBLICATION. (Name(s) of researcher(s): Hiroshi Nishina, Makoto Furutani-Seiki and Hisato Kondoh)
■The RECIPIENT shall include the name(s) of following researcher(s) as co-authors in the first PUBLICATION. (Name(s) of researcher(s): Makoto Furutani-Seiki and Hisato Kondoh)
Category Mutant of embryogeneses
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by ERATO Kondoh Differentiation Signaling Project, Japan Science and Technology Agency
Order Request

ERATO Kondoh Project - 1
Submitter Takao Sasado   Observed in  
Attribute   Value  
Phenotype
Manifestation
recessive

penetrance_complete
expressivity_high
Note  
Comment PGCs formed tight bilateral clumps in wild-type embryos at st. 27. In sakura (sakjr10-4A, j153-3A) mutant embryos, PGCs were dispersed in bilateral longitudinal lines and this occurred in somitic-medoderm area. sak mutation also caused morphogenetic defects, such as small eyes, large tecta, flattened somitic mesoderm and an undulated notochord at st. 27. sak mutation was a recessive and homozygosity caused embryonic lethality.  
Image
 
Preparation
○live 
●whole-mount 
○LM-section 
○EM-section 
○not specified 
Image View Orientation
○side view (lateral) 
●dorsal 
○ventral 
○frontal 
○animal pole 
○vegetal pole 
○transverse 
○sagittal 
○parasagittal 
○not specified 
○anterior to left 
●anterior to top 
○anterior to right 
○dorsal to top 
○dorsal to right 
○oblique 
○not specified 
Caption / Comment
Whole-mount in situ hybridization using the olvas probe at st. 27. In the wild-type embryo (top) PGCs are clustered in the ventrolateral regions of the trunk. In the sak mutant embryo, PGCs are loosely aligned bilaterally, but their locations were more medial than lateral edges of the body at st. 27 (bottom). Broken lines indicate the lateral edges of the body.  


/medaka