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OlTGK2(W368X)
| Strain information | |
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| Strain ID | MT997 |
| Strain Name | OlTGK2(W368X) |
| Strain Type | Mutants |
| Characteristics |
OlTGK2 is a possible medaka orthologue for mammalian skin-type transglutaminase(TG1), which is an essential enzyme for skin formation. The full-lenghth cDNA sequences were encoded as a Olgi48d24 (NBRP database) and characterized for its enzymatic property by producing the recombinant protein. By TILLING methods, from the library we identified a mutant at the site of Trp368 was replaced to stop codon which causes inactive enzyme. As in the case of OlTGK1, larvae stage fish is slightly sensitive to higher osmolality solution, containing 150 mM NaCl in the circulating tap water. Data are published in Biosci. Biotechnol. Biochem. 82, 1165-1168 (2018). |
Journal
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| Special affairs [Address] |
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: WATANABE, Yuko, et al. Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases. Bioscience, biotechnology, and biochemistry, 2018, 1-4.) ■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same. |
| Category | Mutants created by TILLING |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Nagoya University |
| Order |
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| Document (PDF) | Genotyping protocol_OlTGK1 OlTGK2 mutant |
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