acp5-mCherry-Nuc
Strain information | |
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Strain ID | TG1249 |
Strain Name | acp5-mCherry-Nuc |
Strain Type | Transgenics |
Characteristics | acp5 (acid phosphatase 5, tartrate resistant), also known as TRAP, is a glycosylated monomeric metalloprotein enzyme. acp5/TRAP is used as a differentiation marker of osteoclasts. A fosmid clone (GOLWFno144_g15) including the acp5 gene, which was identified from the Medaka ensemble database, was used for homologous recombination. The mCherry-Nuc-kanamycin cassette was recombined into exon 1 of the medaka acp5 gene. The medaka Cab line was used for establishment of the acp5-mCherry-Nuc transgenic medaka. The mCherry expression is observed in nuclei of putative osteoblasts. |
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Special affairs [Address] | ■ The RECIPIENT should make a prior contact with Keiji Inohaya (kinohaya@bio.titech.ac.jp), and arrange for collaborative research or offer conditions with a written form of consent. Then, the RECIPIENT shall make a request for this strain to NBRP Medaka at NIBB. |
Category | Transgenic strains |
Organization | National Institute for Basic Biology |
Live | ○ |
Deposited by | Tokyo Institute of Technology |
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Image |
TG1249_transgene-map |
Image |
TG1249_acp5-mChery-Nuc_Larva Lateral view of tail region of ca. 8.5 mm larva. mCherry-expression is observed in nucleus of putative osteoclast cells. The putative osteoclast cells are located on vertebral arches (arrowheads) and anal fin (arrow). |