With the TALEN technology, seven base pair in Exon2 of DJ-1 (parkinson protein 7, PARK 7) gene has deleted. Because of this frame shift mutation, DJ-1 protein cannot be detected by Western Blotting Analysis.
Ansai S, Inohaya K, Yoshiura Y, Schartl M, Uemura N, Takahashi R, Kinoshita M. Design, evaluation, and screening methods for efficient targeted mutagenesis with transcription activator-like effector nucleases in medaka. Dev Growth Differ 2014/01/01.56(1).98-107.
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: Ansai S, Sakuma T, Yamamoto T, Ariga H, Uemura N, Takahashi R, Kinoshita M. (2013) Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-LikeEffector Nucleases. GENETICS, 193, 739-749.)
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