■The RECIPIENT shall include the name(s) of following researcher(s) as co-authors in the first PUBLICATION. (Name(s) of researcher(s): Hiroshi Nishina, Makoto Furutani-Seiki and Hisato Kondoh)
Category
Mutant of embryogeneses
Organization
National Institute for Basic Biology
Frozen sperm in NIBB
○
Deposited by
ERATO Kondoh Differentiation Signaling Project, Japan Science and Technology Agency
Order
ERATO Kondoh Project
-
1
Submitter
Tomomi Watanabe
Observed in
gall bladder
Attribute
color
Value
abnormal
Phenotype
Manifestation
Note
Comment
Affected the color of bile in the gall bladder. Bile in the wild-type embryo at st. 35 was light green. The bile colors in this mutants were orange. suo mutant embryos hatched and homozygotes were fertile. To determine whether these mutations affect either erythropoiesis or hemoglobin-bile metabolism, we first carried out hemoglobin staining with o-dianisidine. The blood vessels and heart, but not the gall bladder, were stained in suo mutant embryos. a-0, a-1, and b-1. a-0, a-1, and b-1 globins were expressed in suo mutants at st. 33 using in situ hybridization.
●side view (lateral)
○dorsal
○ventral
○frontal
○animal pole
○vegetal pole
○transverse
○sagittal
○parasagittal
○not specified
●anterior to left
○anterior to top
○anterior to right
○dorsal to top
○dorsal to right
○oblique
○not specified
Caption / Comment
Live embryos. The bile colors in this mutants were orange. White arrow heads and white broken lines indicate the gall bladder and margin of the liver, respectively. Black to brownish spots are melanophores on the yolk.
ERATO Kondoh Project
-
2
Submitter
Tomomi Watanabe
Observed in
embryo
Attribute
viability
Value
viable
Phenotype
Manifestation
Note
Comment
Affected the color of bile in the gall bladder. Bile in the wild-type embryo at st. 35 was light green. The bile colors in this mutants were orange. suo mutant embryos hatched and homozygotes were fertile. To determine whether these mutations affect either erythropoiesis or hemoglobin-bile metabolism, we first carried out hemoglobin staining with o-dianisidine. The blood vessels and heart, but not the gall bladder, were stained in suo mutant embryos. a-0, a-1, and b-1. a-0, a-1, and b-1 globins were expressed in suo mutants at st. 33 using in situ hybridization.
