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wnt4b-EGFP
| Strain information | |
|---|---|
| Strain ID | TG1259 |
| Strain Name | wnt4b-EGFP |
| Strain Type | Transgenics |
| Characteristics | Wnt4b is a member of wingless-type MMTV integration site family (WNT). A 3.2 kb genomic fragment encompassing the wnt4b promoter region was cloned into the HindIII-SalI sites of the pEGFP-1 vector (CLONTECH).The EGFP expression is observed in the floor plate cells. |
Journal
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| Special affairs [Address] |
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: Inohaya K, Takano Y, Kudo A. (2010). Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka. Development. 137, 1807-1813.) |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Tokyo Institute of Technology |
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| Image |
TG1259_transgene-map A 3.2kb wnt4b promoter was ligated to HindIII and SalI sites of pEGFP1 (Clontech). |
| Image |
TG1259_wnt4b-EGFP_embryo (A-B) EGFP expresses in the floor plate cells (green). Dorso-lateral view of a day-4 embryo.(A) Bright-field image. (B) Fluorescence image of “A.” (C) Higher magnification of the floor plate cells (green). |
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