A 7 bp was deleted in exon 9 of hsf1 (heat shock transcription factor 1) gene by the TALEN genome editing system. This region contains essential phosphorylation sites required for the activation of Hsf1. The mutation results in a defective Hsf1 function. Because homozygote females are infertile, hsf1 KO medaka were produced by mating KO male fish with heterogenous female fish.
The RECIPIENT shall refer the following publication in any PUBLICATIONS. Furukawa F, Hamasaki S, Hara S, Uchimura T, Shiraishi E, Osafune N, Takagi H, Yazawa T, Kamei Y, Kitano T. Heat shock factor 1 protects germ cell proliferation during early ovarian differentiation in medaka. Sci Rep 2019;9(1):6927. doi: 10.1038/s41598-019-43472-4.
