ukon^j152-8A

Strain information
Strain ID MT438
Strain Name ukon^j152-8A
Strain Type Mutants
Symbol uko^j152-8A
Characteristics Abnormal liver development
Journal
Special affairs [Address] ■The RECIPIENT shall include the name(s) of following researcher(s) as co-authors in the first PUBLICATION. (Name(s) of researcher(s): Hiroshi Nishina, Makoto Furutani-Seiki and Hisato Kondoh)
Category Mutant of embryogeneses
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by ERATO Kondoh Differentiation Signaling Project, Japan Science and Technology Agency
Order Request

ERATO Kondoh Project - 1
Submitter Tomomi Watanabe   Observed in phospholipid metabolism  
Attribute behavioral_activity   Value impaired  
Phenotype
Manifestation
recessive

penetrance_complete
expressivity_complete
Note  
Comment The fluorescence intensities of PED6 metabolites markedly decreased at st. 35. Mutants swallowed the unquenched form of PED6, and the gut, liver and gall bladder showed fluorescence as wild-type embryos. Mutant embryos appeared normal in the morphologies of the liver, gall bladder and intestine at st. 35. Mutant embryos could hatch but their larvae died a few days after hatching.  
Image
 
Preparation
●live 
○whole-mount 
○LM-section 
○EM-section 
○not specified 
Image View Orientation
●side view (lateral) 
○dorsal 
○ventral 
○frontal 
○animal pole 
○vegetal pole 
○transverse 
○sagittal 
○parasagittal 
○not specified 
●anterior to left 
○anterior to top 
○anterior to right 
○dorsal to top 
○dorsal to right 
○oblique 
○not specified 
Caption / Comment
Laterl view, anterior to the left. Live embryos stained with PED6 at st. 35. Metabolized PED6 can been seen as a green emission (Em: 505 nm) in the gall bladder (white arrowheads). Mutations affect lipid metabolism.  

ERATO Kondoh Project - 2
Submitter Tomomi Watanabe   Observed in embryo  
Attribute structure   Value degenerate  
Phenotype
Manifestation
recessive

penetrance_complete
expressivity_complete
Note  
Comment The fluorescence intensities of PED6 metabolites markedly decreased at st. 35. Mutants swallowed the unquenched form of PED6, and the gut, liver and gall bladder showed fluorescence as wild-type embryos. Mutant embryos appeared normal in the morphologies of the liver, gall bladder and intestine at st. 35. Mutant embryos could hatch but their larvae died a few days after hatching.  
Image
 
Preparation
●live 
○whole-mount 
○LM-section 
○EM-section 
○not specified 
Image View Orientation
○side view (lateral) 
●dorsal 
○ventral 
○frontal 
○animal pole 
○vegetal pole 
○transverse 
○sagittal 
○parasagittal 
○not specified 
●anterior to left 
○anterior to top 
○anterior to right 
○dorsal to top 
○dorsal to right 
○oblique 
○not specified 
Caption / Comment
Live embryos from dorsal views. Mutant embryos show degenerative.  

ERATO Kondoh Project - 3
Submitter Tomomi Watanabe   Observed in embryo  
Attribute viability   Value lethal  
Phenotype
Manifestation
recessive

penetrance_complete
expressivity_complete
Note  
Comment The fluorescence intensities of PED6 metabolites markedly decreased at st. 35. Mutants swallowed the unquenched form of PED6, and the gut, liver and gall bladder showed fluorescence as wild-type embryos. Mutant embryos appeared normal in the morphologies of the liver, gall bladder and intestine at st. 35. Mutant embryos could hatch but their larvae died a few days after hatching.  
Image
 
Preparation
○live 
○whole-mount 
○LM-section 
○EM-section 
●not specified 
Image View Orientation
○side view (lateral) 
○dorsal 
○ventral 
○frontal 
○animal pole 
○vegetal pole 
○transverse 
○sagittal 
○parasagittal 
●not specified 
○anterior to left 
○anterior to top 
○anterior to right 
○dorsal to top 
○dorsal to right 
○oblique 
●not specified 
Caption / Comment
 


/medaka