osc:eGFP

Strain information
Strain ID TG1133
Strain Name osc:eGFP
Strain Type Transgenics
Characteristics Single transgenic line labelling osteocalcin (osc) expressing osteoblasts with GFP.
Transgene: The osteocalcin is a secretory protein found exclusively in bone tissue. It is secreted by mature osteoblasts in medaka; used as a terminal differentiation marker of osteogenesis in mammals. The transgenic line construct was made by cloning a genomic fragment (3.7k bp) encompassing the osteocalcin promoter region from the genomic DNA of the medaka in front of EGFP into a vector with flanking I-SceI meganuclease restriction sites.
Journal
  •    2  Hits.
Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: RENN, Joerg; WINKLER, Christoph. Osterix‐mCherry transgenic medaka for in vivo imaging of bone formation. Developmental Dynamics, 2009, 238.1: 241-248.
TO, Thuy Thanh, et al. Rankl-induced osteoclastogenesis leads to loss of mineralization in a medaka osteoporosis model. Development, 2012, 139.1: 141-150
RENN, Jörg, et al. A col10a1: nlGFP transgenic line displays putative osteoblast precursors at the medaka notochordal sheath prior to mineralization. Developmental biology, 2013, 381.1: 134-143.)
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
Category Transgenic strains
Organization National Institute for Basic Biology
Live
Deposited by National University of Singapore
Order Request
Image

TG1133 osc-EGFP 9 dph
Lateral view of osc:eGFP medaka at 9 days post hatching. EGFP expression is seen in mature osteoblasts of cranial bones and vertebral column.
Document (PDF) TG1133 osc-EGFP plasmid map


/medaka