GFP expression was observed at neural crest cells and moter neuron axons from St30. Transgene: 3kb FoxD3 promoter was ligated to Hind3 and BamH1 site of pBSKI2-GFP (AmpR).
Notes
Cab-Tg(FoxD3-GFP) (TG874) and Cab-Tg(FoxD3-GFP)2 (TG1068) are independent stable transgenic lines. Ishikawa et al. (2015) used the Cab-Tg(FoxD3-GFP)2 (TG1068) for a research of the pineal complex.
Ishikawa Y, Inohaya K, Yamamoto N, Maruyama K, Yoshimoto M, Iigo M, Oishi T, Kudo A, Ito H. The Parapineal Is Incorporated into the Habenula during Ontogenesis in the Medaka Fish. Brain Behav Evol 2015/01/01.85(4).257-70.
Category
Transgenic strains
Organization
National Institute for Basic Biology
Frozen sperm in NIBB
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Deposited by
Tokyo Institute of Technology
Order
Image
GFP expression pattern of foxD3-gfp transgenic medakas
Latral view of the medaka larva at st40. GFP expression is observed at the neural crest cells and glial cells of putative motor neural axon.