NBRP Medaka

Medaka Strain
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Tg(Actb-SLa:GFP)

Strain information
Strain ID	TG1147
Strain Name	Tg(Actb-SLa:GFP)
Strain Type	Transgenics
Characteristics	Fish of this strain show darkend skin color due to ectopic overexpression of somatolactin alpha. The transgene also expresses GFP as a reporter.
Journal	7 Hits. PMID: 32903371 [ RRC reference ] Kanazawa N, Goto M, Harada Y, Takimoto C, Sasaki Y, Uchikawa T, Kamei Y, Matsuo M, Fukamachi S. Changes in a Cone Opsin Repertoire Affect Color-Dependent Social Behavior in Medaka but Not Behavioral Photosensitivity. Front Genet 2020/08/12.11.801. PMID: 29447852 [ RRC reference ] Kamijo M, Kawamura M, Fukamachi S. Loss of red opsin genes relaxes sexual isolation between skin-colour variants of medaka. Behav Processes 2018/05/01.150.25-28. PMID: 28202469 [ RRC reference ] Ikawa M, Ohya E, Shimada H, Kamijo M, Fukamachi S. Establishment and maintenance of sexual preferences that cause a reproductive isolation between medaka strains in close association. Biol Open 2017/02/15.6(2).244-251. PMID: 19788724 [ RRC reference ] Fukamachi S, Kinoshita M, Aizawa K, Oda S, Meyer A, Mitani H. Dual control by a single gene of secondary sexual characters and mating preferences in medaka. BMC Biol 2009/09/29.7.64. PMID: 28166717 [ RRC reference ] Homma N, Harada Y, Uchikawa T, Kamei Y, Fukamachi S. Protanopia (red color-blindness) in medaka: a simple system for producing color-blind fish and testing their spectral sensitivity. BMC Genet 2017/02/06.18(1).10. PMID: 27260680 [ RRC reference ] Utagawa U, Higashi S, Kamei Y, Fukamachi S. Characterization of assortative mating in medaka: Mate discrimination cues and factors that bias sexual preference. Horm Behav 2016/08/01.84.9-17. PMID: 19393303 [ RRC reference ] Fukamachi S, Yada T, Meyer A, Kinoshita M. Effects of constitutive expression of somatolactin alpha on skin pigmentation in medaka. Gene 2009/08/01.442(1-2).81-7.
Special affairs　[Address]	■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: Fukamachi, Shoji, et al. "Effects of constitutive expression of somatolactin alpha on skin pigmentation in medaka." Gene 442.1 (2009): 81-87. )
Category	Transgenic strains
Organization	National Institute for Basic Biology
Frozen sperm in NIBB	○
Deposited by	Japan Women's University, Kyoto University
Order
Image	TG1147_Whole Body Adult fish of Tg(Actb-SLa:GFP)(left) and (ci (MT15))(right).
Image	TG1147_Tg(Actb-SLa GFP)_Transgene_map Medaka beta-actin promoter is connected to medaka somatolactin-alpha cDNA. The IRES and ORF of hrGFP are connected in its downstream.