Tg(pnp4a-hs:GFP)
Strain information | |
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Strain ID | TG1230 |
Strain Name | Tg(pnp4a-hs:GFP) |
Strain Type | Transgenics |
Characteristics | GFP expression was observed in the eye and abdomen at Juvenal stage. Genotype of the original strain is pnp4a^hs:GFP/gu. Knock-in allele is non-functional and homozygote with transgene shows the less-pigmented iridophores. |
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Special affairs [Address] |
■ 利用者は、すべての本件刊行物において、次の文献を引用する。 (文献名:WATAKABE, Ikuko, et al. Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering. Zoological Letters (2018) 4:3) |
Category | Transgenic strains |
Organization | National Institute for Basic Biology |
Frozen sperm in NIBB | ○ |
Deposited by | National Institute for Basic Biology |
Order | |
Image |
medaka_forward_reverse_genetics Knock in at the 5' NTR region of pnp4a gene with Tbait:hs:GFP donor vector. |
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Transgene map_Tbait-hs-GFP |
Image |
TG1230_Tg(pnp4a-hs GFP)_embryo The progenies (4 dpf) of a pnp4a-transgenic founder crossed to an existing gu (MT58) mutant. All GFP-positive F1 fish have less-pigmented eyes and all GFP-negative F1 fish have wild-type pigmentation. |