OlTGK1(ins2)

Strain information
Strain ID MT1118
Strain Name OlTGK1(ins2)
Strain Type Mutants
Characteristics In the sequence of OlTGK1, 3 bp in the sequence (No.341-343, LC068829) was deleted and unrelated sequences (5 bps, CCGTC) were inserted. Totally, 2 bps were inserted resulting in the mutation at the amino acid position No.114 (BAT61949).
At the larvae stage, this mutants is shown to be sensitive to higher osmolality solution, containing 150 mM NaCl in the circulating tap water. They survive less period (0-1 days) that wild-type (2-3 days). Data are published in Biosci. Biotechnol. Biochem. 82, 1165-1168 (2018).
Journal
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Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: WATANABE, Yuko, et al. Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases. Bioscience, biotechnology, and biochemistry, 2018, 1-4.)
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same.
Category Mutants created by genome editing
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by Nagoya University
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Document (PDF) Genotyping protocol_OlTGK1 OlTGK2 mutant


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