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T5-Tg(olphsp70.1-hRluc-Venus)
| Strain information | |
|---|---|
| Strain ID | TG908 |
| Strain Name | T5-Tg(olphsp70.1-hRluc-Venus) |
| Strain Type | Transgenics |
| Symbol | unkown |
| Characteristics | Inducible expresssion of hRluca-Venus was observed in embryos under the fluorescence microscope. After heat shock (42C, 1min), adult fishes show Venus fluorescence in most tissues and organs, except for germ cells. A transgenic embryos and adult fishes expressed Venus in the lens without heat shock. Transgene: 3.4kb medaka heat shock promoter was ligated to codon-humanized Renilla reniformis luciferase (Promega) - Venus (Nagai et al., 2002) fusion gene in pGL4.70 [hRluc] (Promega). |
| Notes | About 10% of this transgenics have malformations such as small one eye. |
Journal
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| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Live | ○ |
| Deposited by | The University of Tokyo |
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| Image |
TG908_T5-Tg(olphsp70.1-hRluc-Venus)_embryo Heat shock (42C for 10 min) induced Venus expression in embryos. Images taken from the same sample under a fluorescence microscope (Left panel) and a bright-field microscope (Right panel) are shown. |
| Image |
TG908_T5-Tg(olphsp70.1-hRluc-Venus)_adult Heat shock (42C for 10 min) induced Venus expression in adult fish throughout the entire body. Images taken from the same sample under a bright-field microscope (Left panel) and a fluorescence microscope (Right panel) are shown. The upper fish is transgenic and the lower fish is wild type. |
| Image |
TG908_T5-Tg(olphsp70.1-hRluc-Venus) transgene map |
2 Hits.
