shimogamo^j41-13D

Strain information
Strain ID MT427
Strain Name shimogamo^j41-13D
Strain Type Mutants
Symbol smg^j41-13D
Characteristics Abnormal PGC distribution/number
Journal
Special affairs [Address] ■The RECIPIENT shall include the name(s) of following researcher(s) as co-authors in the first PUBLICATION. (Name(s) of researcher(s): Takao Sasado, Makoto Furutani-Seiki and Hisato Kondoh)
Category Mutant of embryogeneses
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by ERATO Kondoh Differentiation Signaling Project, Japan Science and Technology Agency
Order Request

ERATO Kondoh Project - 1
Submitter Takao Sasado   Observed in  
Attribute   Value  
Phenotype
Manifestation
recessive

penetrance_complete
expressivity_complete
Note  
Comment PGCs formed tight bilateral clumps in wild-type embryos at st. 27. In shimogamo (smgj41-13D) mutant embryos, however, PGCs did not coalesce but were loosely aligned in bilateral rows in the region more anterior than normal PGC clusters. The homozygous smg mutation caused lethality and other morphogenetic defects such as small lenses at st. 26, an increased space between the lens and posterior retina at st. 26, and cystic pronephric ducts from 3 dpf (days postfertilization).  
Image
 
Preparation
○live 
●whole-mount 
○LM-section 
○EM-section 
○not specified 
Image View Orientation
○side view (lateral) 
●dorsal 
○ventral 
○frontal 
○animal pole 
○vegetal pole 
○transverse 
○sagittal 
○parasagittal 
○not specified 
○anterior to left 
●anterior to top 
○anterior to right 
○dorsal to top 
○dorsal to right 
○oblique 
○not specified 
Caption / Comment
Whole-mount in situ hybridization using the olvas probe at st. 27. In the wild-type embryo (top), PGCs are clustered at the ventrolateral regions of the trunk (indicated by black arrowheads) at the axial level where the posterior trunk detach from the yolk. In the mutant embryo (bottom), PGCs are dispersed along the bilateral lines (broken lines) anterior to the sites of normal PGC clustering. White arrowheads indicate the pigment cells.  


/medaka