SWS2^-4a-2b

Strain information
Strain ID MT1163
Strain Name SWS2^-4a-2b
Strain Type Mutants
Symbol -4a-2b
Characteristics Two frameshift mutations (a four-base deletion and a two-base deletion) was induced by the CRISPR/Cas9 system on the short wavelength sensitive type 2a (SWS2a) and SWS2b opsin genes, respectively. Body-color preferences during mate choice are relaxed, possibly due to a problem in color discrimination. Blue-light sensitivity is normal as the wild-type. There were no differences in phenotypes between sws2a single mutant, sws2b single mutant and double mutant for sws2a and sws2b as the result of the experiment on Kanazawa et al. 2020.
Notes Background of this mutant is ci (MT15) or Actb-SLa:GFP (TG1147), but we use OK-Cab (MT830) embryos in artificially- insemination. Please note that we will use OK-Cab (MT830) for artificially insemination with frozen sperm of this strain.
Journal
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Category Mutants created by genome editing
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by Japan Women's University
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Document (PDF) Genotyping protocol_sws2 mutants


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